DGGE Book Chapter
Dear Friends -
While it may seem that I haven't posted much lately, I have still been working on DGGE-related matters. Together with my colleagues Mary Beth Leigh and Josh Neufeld, I have written a book chapter entitled "Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis". It has some background on the method, as well as detailed protocols for producing gels, primer sets for bacterial, archaeal and fungal community analysis, a list of all required reagents, comparison of three major DGGE makers, and troubleshooting. If this chapter is useful to you, please cite the following:
Green, S.J., Leigh, M.B. and Neufeld, J.D. 2009. Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis. Pages 4137-4158 in: Timmis, K.N. (Ed)Handbook of Hydrocarbon and Lipid Microbiology. Springer (Heidelberg, Germany).
Currently, the chapter is available for download. There may be some slight differences between this and the final typeset chapter, but this should be marginal.