New NCBI BLAST function for primer design
I thought this would be of interest to the community. The BLAST site at NCBI now has a specific page for the design and testing of PCR primers (click here). It looks to be quite sophisticated if necessary (there is a pull down menu for more advanced options).
http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_LOC=BlastHomeAd
posted by Stefan J. Green @ 8:32 AM
3 comments
3 Comments:
Is it possible for you to add a search function in your blog? I am trying to trouble shoot fuzzy bands and would like to be able to search for keywords.
Thanks
PS If there is a search function that I have missed please feel free to point it out!
Hi Sam -
I will see about adding a search function. For the moment, if you open the website in Firefox, you can search by "Control-F" (for find) and type in the term you are looking for.
Cheers,
Stefan
The primers should have similar melting temperatures since annealing in a PCR occurs for both simultaneously.It is very good for knowledge.Thanks
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