New NCBI BLAST function for primer design
I thought this would be of interest to the community. The BLAST site at NCBI now has a specific page for the design and testing of PCR primers (click here). It looks to be quite sophisticated if necessary (there is a pull down menu for more advanced options).
http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_LOC=BlastHomeAd